Author: Ali Punjani; Haowei Zhang; David J. Fleet
Title: Non-uniform refinement: Adaptive regularization improves single particle cryo-EM reconstruction Document date: 2019_12_16
ID: bqwmx5dy_65
Snippet: A beta version of the non-uniform refinement algorithm was released in previous versions of cryoSPARC [22] . Indeed, it has been already been successful in helping to resolve a number of interesting new structures. Examples include a structure of FACT manipulating the nucleasome [19] , multiple conformations of an ABC exporter [14] , mTORC1 docked on the lysosome [24] , the respiratory syncytial virus polymerase complex [9] , a GPCR-G protein-β-.....
Document: A beta version of the non-uniform refinement algorithm was released in previous versions of cryoSPARC [22] . Indeed, it has been already been successful in helping to resolve a number of interesting new structures. Examples include a structure of FACT manipulating the nucleasome [19] , multiple conformations of an ABC exporter [14] , mTORC1 docked on the lysosome [24] , the respiratory syncytial virus polymerase complex [9] , a GPCR-G protein-β-arrestin megacomplex [20] , and MERS-CoV/SARS-CoV with neutralizing antibodies [32] . An updated non-uniform refinement algorithm implementation, as described in this work, will be released in an upcoming version of cryoSPARC (www.cryosparc.com).
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