Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation

Myra Hosmillo; Jia Lu; Michael R. McAllaster; James B. Eaglesham; Xinjie Wang; Edward Emmott; Patricia Domingues; Yasmin Chaudhry; Timothy J Fitzmaurice; Matthew K.H. Tung; Marc Panas; Gerald McInerney; Nicholas Locker; Craig B. Willen; Ian Goodfellow

in the viral life cycle (Fig 7C) . Transfection of VPg-linked RNA into the ΔG3BP1 cell 260 lines reconstituted with WT G3BP1 restored the ability to produce infectious virus 261 ( Fig 7B) and the production of viral proteins (Fig 7D) . A minor increase in viral 262 infectivity was observed in the ΔG3BP1 cell line reconstituted with the ΔRGG 263 construct producing viral titres that were higher than those obtained from the WT 264 complemented line in the presence of 2CMC, suggesting low levels of viral 265 replication ( Fig 7B) . However, the levels of viral proteins produced in this line was 266 below the limit of detection by western blot (Fig 7D) . These data confirm that G3BP1 267 is required for a post entry stage of the norovirus life cycle and that in the absence of 268 G3BP1 only residual norovirus replication is observed. 269