Author: Myra Hosmillo; Jia Lu; Michael R. McAllaster; James B. Eaglesham; Xinjie Wang; Edward Emmott; Patricia Domingues; Yasmin Chaudhry; Timothy J Fitzmaurice; Matthew K.H. Tung; Marc Panas; Gerald McInerney; Nicholas Locker; Craig B. Willen; Ian Goodfellow
Title: Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation Document date: 2019_3_8
ID: d0q5lhf4_23
Snippet: To define the precise role of G3BP1 in the early stages of the virus life cycle, we 272 used strand-specific RT-qPCR to quantify the levels of viral positive and negative 273 sense RNA in WT and ΔG3BP1 cell lines following infection with MNV. As a control, 274 2CMC was included following virus inoculation as illustrated in the experimental time 275 line ( Fig 8A) . The production of viral positive sense RNA was reduced to background 276 levels i.....
Document: To define the precise role of G3BP1 in the early stages of the virus life cycle, we 272 used strand-specific RT-qPCR to quantify the levels of viral positive and negative 273 sense RNA in WT and ΔG3BP1 cell lines following infection with MNV. As a control, 274 2CMC was included following virus inoculation as illustrated in the experimental time 275 line ( Fig 8A) . The production of viral positive sense RNA was reduced to background 276 levels in the absence of G3BP1, comparable to levels observed when the 2CMC was 277 present during the infection (Fig 8B) . Viral negative sense RNA synthesis was also 278 reduced to below the detection limit of the assay in ΔG3BP1 cell lines ( Fig 8C) . 279
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