Author: Jacob E. Choby; Hanna B. Buechi; Allison J. Farrand; Eric P. Skaar; Matthew F. Barber
Title: Molecular basis for the evolution of species-specific hemoglobin capture by pathogenic Staphylococcus Document date: 2018_6_7
ID: ieh5cvw1_55
Snippet: High-Fidelity Master Mix (Thermo); cells were treated with 20 µg of lysostaphin (AMBI Products, 427 Lawrence, NY) and DNA wasisolated with Wizard genomic DNA extraction kit (Promega, Madison, WI). 428 S. aureus isdB (NWMN_1040) was amplified using primers JC343/344, S. schweitzeri isdB 429 (ERS140239_01018) using primers JC218/219, and S. argenteus isdB (SAMSHR1132_09750) using 430 primers JC216/217. Each primer pair included homology to pOS1 P .....
Document: High-Fidelity Master Mix (Thermo); cells were treated with 20 µg of lysostaphin (AMBI Products, 427 Lawrence, NY) and DNA wasisolated with Wizard genomic DNA extraction kit (Promega, Madison, WI). 428 S. aureus isdB (NWMN_1040) was amplified using primers JC343/344, S. schweitzeri isdB 429 (ERS140239_01018) using primers JC218/219, and S. argenteus isdB (SAMSHR1132_09750) using 430 primers JC216/217. Each primer pair included homology to pOS1 P lgt digested with NdeI and BamHI-HF 431 All rights reserved. No reuse allowed without permission.
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