Author: Xiankun Zeng; Jun Liu; April Babka; Brian Kearney; Sheli Radoshitzky; Jens Kuhn
Title: Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens Document date: 2020_4_22
ID: nzwe7hpg_30
Snippet: Because SARS-CoV-2-infected animal tissues were not available at the time of this study, we 158 were restricted to evaluate FFPE pellets of Vero 76 cells as a surrogate. However, we prepared 159 FFPE cell pellets using the same process used for FFPE tissues preparation. Additionally, FFPE 160 cell pellets have been widely used to evaluate antibodies and ISH assays and other reagents for 161 FFPE tissue analysis and have been largely predictive of.....
Document: Because SARS-CoV-2-infected animal tissues were not available at the time of this study, we 158 were restricted to evaluate FFPE pellets of Vero 76 cells as a surrogate. However, we prepared 159 FFPE cell pellets using the same process used for FFPE tissues preparation. Additionally, FFPE 160 cell pellets have been widely used to evaluate antibodies and ISH assays and other reagents for 161 FFPE tissue analysis and have been largely predictive of reactivity with genuine tissues (33, 37, 162 38). We are therefore confident that the SARS-CoV-2 IHC, ISH, mFISH, and dual staining 163 assays we developed and characterized will be highly useful to study pathogenesis of SARS-
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