Author: Changhai Lei; Wenyan Fu; Kewen Qian; Tian Li; Sheng Zhang; Min Ding; Shi Hu
Title: Potent neutralization of 2019 novel coronavirus by recombinant ACE2-Ig Document date: 2020_2_2
ID: aeuy92bx_12
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.01.929976 doi: bioRxiv preprint method 14 with immobilized anti-human Fc polyclonal antibody on a CM5 chip (~150 RU) to capture the fusion proteins and and then injected CoV RBDs (12.5 nM~200 nM). blank flow cells were used for the correction of the binding response. The surface plasmon resonance (SPR) method with a BIAcore-2000 was used .....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.01.929976 doi: bioRxiv preprint method 14 with immobilized anti-human Fc polyclonal antibody on a CM5 chip (~150 RU) to capture the fusion proteins and and then injected CoV RBDs (12.5 nM~200 nM). blank flow cells were used for the correction of the binding response. The surface plasmon resonance (SPR) method with a BIAcore-2000 was used to measure the monovalent binding affinity of the fusion protein and performed kinetic analysis using a 1:1 L model that simultaneously fit ka and kb.
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