Selected article for: "chain reaction and detection classification"
Author: David A Wilkinson; Lea Joffrin; Camille Lebarbenchon; Patrick Mavingui
Title: Partial RdRp sequences offer a robust method for Coronavirus subgenus classification
  • Document date: 2020_3_6
    • ID: jjraqr85_1
    Snippet: Importance Statement: 28 The analysis of polymerase chain reaction amplicons derived from biological samples is the most 29 common modern method for detection and classification of infecting viral agents, such as 30 Coronaviruses . Recent updates to the official standard for taxonomic classification of Coronaviruses, 31 however, may leave researchers unsure as to whether the viral sequences they obtain by these 32 methods can be classified into s.....
    Document: Importance Statement: 28 The analysis of polymerase chain reaction amplicons derived from biological samples is the most 29 common modern method for detection and classification of infecting viral agents, such as 30 Coronaviruses . Recent updates to the official standard for taxonomic classification of Coronaviruses, 31 however, may leave researchers unsure as to whether the viral sequences they obtain by these 32 methods can be classified into specific viral taxa due to variations in the sequences when compared 33 to type strains. Here, we present a plausible method for defining genetic dissimilarity cut-offs that 34 will allow researchers to state which taxon their virus belongs to and with what level of certainty. To 35 assist in this, we also provide the R package 'MyCoV' which classifies user generated sequences. 36 author/funder. All rights reserved. No reuse allowed without permission.

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