Author: Roni Ben-Ami; Agnes Klochendler; Matan Seidel; Tal Sido; Ori Gurel-Gurevich; Moran Yassour; Eran Meshorer; Gil Benedek; Irit Fogel; Esther Oiknine-Djian; Asaf Gertler; Zeev Rotstein; Bruno Lavi; Yuval Dor; Dana G Wolf; Maayan Salton; Yotam Drier
Title: Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection Document date: 2020_4_22
ID: 5s03f0pp_3
Snippet: Several attempts to address this challenge have already been suggested, that can be categorized into three major approaches. The first approach is to replace PCR based methods by other direct diagnostic methods such as Loopmediated Isothermal Amplification (LAMP) [1] [2] [3] and CRISPR based diagnostic tools [4] [5] [6] , the second approach involves serological surveys [7] [8] [9] [10] , and the third approach involves the improvement of the PCR.....
Document: Several attempts to address this challenge have already been suggested, that can be categorized into three major approaches. The first approach is to replace PCR based methods by other direct diagnostic methods such as Loopmediated Isothermal Amplification (LAMP) [1] [2] [3] and CRISPR based diagnostic tools [4] [5] [6] , the second approach involves serological surveys [7] [8] [9] [10] , and the third approach involves the improvement of the PCR methods capacity by optimization and automation (e.g. [11] [12] [13] ) or by reducing the required number of tests via pooling samples together, known as group testing.
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