Author: Mauricio J Farfan; Juan P Torres; Miguel ORyan; Mauricio Olivares; Pablo Gallardo; Carolina Salas
Title: Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing Document date: 2020_4_17
ID: kuqinczz_7
Snippet: Nucleic acid extraction. Nucleic acids extraction of 400 µl of the pool of samples was performed using: a) MagNA Pure Compact Nucleic Acid Isolation Kit I (automated extraction) on the MagNA Pure LC instrument or b) High Pure Viral Nucleic Acid Kit (manual extraction), according to the manufacturer's instructions (Roche). The elution volume was set to 50 µl. We also performed RT-qPCR without extraction, adding 5 µl of the pool samples directly.....
Document: Nucleic acid extraction. Nucleic acids extraction of 400 µl of the pool of samples was performed using: a) MagNA Pure Compact Nucleic Acid Isolation Kit I (automated extraction) on the MagNA Pure LC instrument or b) High Pure Viral Nucleic Acid Kit (manual extraction), according to the manufacturer's instructions (Roche). The elution volume was set to 50 µl. We also performed RT-qPCR without extraction, adding 5 µl of the pool samples directly to the RT-PCR reaction.
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