Selected article for: "assembly viral replication and coronavirus infection"
Author: Li, Shuang; Zhao, Qi; Zhang, Yinjie; Zhang, Yang; Bartlam, Mark; Li, Xuemei; Rao, Zihe
Title: New nsp8 isoform suggests mechanism for tuning viral RNA synthesis
  • Cord-id: gtajz6jr
  • Document date: 2010_2_1
    • ID: gtajz6jr
    Snippet: During severe acute respiratory syndrome coronavirus (SARS-CoV) infection, the activity of the replication/transcription complexes (RTC) quickly peaks at 6 hours post infection (h.p.i) and then diminishes significantly in the late post-infection stages. This “down-up-down” regulation of RNA synthesis distinguishes different viral stages: primary translation, genome replication, and finally viron assembly. Regarding the nsp8 as the primase in RNA synthesis, we confirmed that the proteolysis p
    Document: During severe acute respiratory syndrome coronavirus (SARS-CoV) infection, the activity of the replication/transcription complexes (RTC) quickly peaks at 6 hours post infection (h.p.i) and then diminishes significantly in the late post-infection stages. This “down-up-down” regulation of RNA synthesis distinguishes different viral stages: primary translation, genome replication, and finally viron assembly. Regarding the nsp8 as the primase in RNA synthesis, we confirmed that the proteolysis product of the primase (nsp8) contains the globular domain (nsp8C), and indentified the resectioning site that is notably conserved in all the three groups of coronavirus. We subsequently crystallized the complex of SARS-CoV nsp8C and nsp7, and the 3-D structure of this domain revealed its capability to interfuse into the hexadecamer super-complex. This specific proteolysis may indicate one possible mechanism by which coronaviruses to switch from viral infection to genome replication and viral assembly stages.

    Search related documents:
    Co phrase search for related documents
    • acid affinity and low molecular: 1
    • acid binding and activity interfere: 1
    • acid binding and activity responsible: 1, 2
    • acid binding and acute sars cov respiratory syndrome coronavirus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • acid binding and low molecular: 1
    • activity interfere and acute sars cov respiratory syndrome coronavirus: 1, 2, 3, 4
    • activity responsible and acute sars cov respiratory syndrome coronavirus: 1, 2
    • activity responsible and low molecular: 1
    • acute sars cov respiratory syndrome coronavirus and loading dye: 1
    • acute sars cov respiratory syndrome coronavirus and low molecular: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25