Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases

Mandy Muller; Britt A. Glaunsinger

Using an in vitro RNA-pulldown based strategy, we previously determined that the 261 IL-6 SRE assembles a specific ribonucleoprotein (RNP) 'escape' complex that is critical for 262 its ability to mediate protection from cleavage by SOX [26, 27] . Given the partial similarities 263 in length, sequence, and structure between the IL-6 SRE and the G-SRE, we hypothesized 264 that they might also assemble a similar set of RNPs to mediate their protective function. ChIRP-MS for the IL-6 3' UTR and were similarly associated with the GADD45B 3' UTR 277 (Table S2 & Fig. 5B , purple nodes). We sought to independently validate a subset of these 278 common interactors by western blot following ChIRP (Fig. 5C) . Again, we recovered NCL 279