Author: Chang, Mau-Sun; Lu, Yen-Ta; Ho, Shin-Tsung; Wu, Chao-Chih; Wei, Tsai-Yin; Chen, Chia-Ju; Hsu, Yun-Ting; Chu, Po-Chen; Chen, Ching-Hsin; Chu, Jien-Ming; Jan, Ya-Lin; Hung, Chia-Chien; Fan, Chi-Chen; Yang, Yuh-Cheng
Title: Antibody detection of SARS-CoV spike and nucleocapsid protein Cord-id: lob26ih1 Document date: 2004_2_20
ID: lob26ih1
Snippet: Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodie
Document: Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6× His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV.
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