Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus Document date: 2018_9_23
ID: n5liudlg_17
Snippet: To functionally verify the bipartite signal processor, reverse transcription LAMP assays containing the degenerate CAN3.2GO or the NS1NS5.2GO probes were supplemented with primers specific to either ZIKV NS1, NS3, NS5, or CA genes. At amplification endpoint, fluorescence of CAN3.2GO probe increased only in assays containing CA or N3 amplicons ( Figure 6A ). Meanwhile the N1N5.2GO probe was activated only in the presence of NS1 or NS5 sequences. B.....
Document: To functionally verify the bipartite signal processor, reverse transcription LAMP assays containing the degenerate CAN3.2GO or the NS1NS5.2GO probes were supplemented with primers specific to either ZIKV NS1, NS3, NS5, or CA genes. At amplification endpoint, fluorescence of CAN3.2GO probe increased only in assays containing CA or N3 amplicons ( Figure 6A ). Meanwhile the N1N5.2GO probe was activated only in the presence of NS1 or NS5 sequences. Both probes remained quenched in the presence of non-specific LAMP amplicons. Moreover, amplicon-specific 2GO probe fluorescence could be readily distinguished from non-specific noise simply by direct visual examination of assay tubes or their cellphone images ( Figure 6B ).
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