Potential host range of multiple SARS-like coronaviruses and an improved ACE2-Fc variant that is potent against both SARS-CoV-2 and SARS-CoV-1

Yujun Li; Haimin Wang; Xiaojuan Tang; Danting Ma; Chengzhi Du; Yifei Wang; Hong Pan; Qing Zou; Jie Zheng; Liangde Xu; Michael Farzan; Guocai Zhong

, and SARS-CoV-1. a, 293T cells were transfected with a vector control plasmid, an ACE2 expression plasmid, or ACE2 and TMPRSS2 expression plasmids. The cells were then infected with GFP reporter retroviruses pseudotyped with SARS-CoV-2 and SARS-CoV-1 spike proteins, respectively. Spike protein-mediated coronavirus entry was detected by fluorescent microscopy at 48 hours post infection. b, Experiments similar to a except that retroviruses carrying a luciferase reporter gene and pseudotyped with SARS-CoV-2, SARS-CoV-1 spike mutants and VSV-G were used. Coronavirus spike mutant-mediated viral entry was measured by the luciferase reporter at 48 hours post infection. c, 293T cells expressing the indicated ACE2 orthologs were infected with luciferase-reporter retroviruses pseudotyped with the indicated spike proteins. ACE2 orthologmediated viral entry was measured by the luciferase reporter at 48 hours post infection. Original reporter expression data are provided in Extended Data Figure 3b . Relative infection (%) values for each ACE2 ortholog-mediated viral entry were independently calculated against the highest expression values of the same pseudotype panel and presented as a heatmap according to the indicated color code. Data shown are representative of three experiments independently performed by three different people with similar results, and data points represent mean ± s.d. of three biological replicates. author/funder. All rights reserved. No reuse allowed without permission.