Author: Monica Sentmanat; Evguenia Kouranova; Xiaoxia Cui
Title: One-step RNA extraction for RT-qPCR detection of 2019-nCoV Document date: 2020_4_5
ID: ihhu7nef_17
Snippet: Samples were incubated in the QE buffer at 65°C for 15 minutes, followed by 98°C for 2 min. Column purification was performed using Qiagen RNeasy Mini Kit (Cat. No. 74104) from 120 ul of each sample, using an elution volume of 30 ul......
Document: Samples were incubated in the QE buffer at 65°C for 15 minutes, followed by 98°C for 2 min. Column purification was performed using Qiagen RNeasy Mini Kit (Cat. No. 74104) from 120 ul of each sample, using an elution volume of 30 ul.
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