Author: Monica Sentmanat; Evguenia Kouranova; Xiaoxia Cui
Title: One-step RNA extraction for RT-qPCR detection of 2019-nCoV Document date: 2020_4_5
ID: ihhu7nef_3
Snippet: All positive control counterpart samples had a Ct value for N1 between 29-32, while all experimental samples had no detectable amplification from the N1 primer/probe set ( Figure 1 ). We detected RNaseP reference signals in both the OP and human cell line extraction controls, which confirms that the extractions were successful. Although an equal volume of lysis extract was used to generate the positive control counterpart of OP experimental sampl.....
Document: All positive control counterpart samples had a Ct value for N1 between 29-32, while all experimental samples had no detectable amplification from the N1 primer/probe set ( Figure 1 ). We detected RNaseP reference signals in both the OP and human cell line extraction controls, which confirms that the extractions were successful. Although an equal volume of lysis extract was used to generate the positive control counterpart of OP experimental samples, RNaseP reference signals were 2 cycles greater for positive controls, suggesting that the swab containing portion retained more cells. But it's important to point out that the raw Ct values do not change the interpretation of results for what is intended to be a qualitative yes/no assay -that the positive and extraction controls performed within the range (<40) to determine the presence or absence of 2019-nCoV N gene in experimental samples 6 .
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