G-quadruplex stabilization in the ions and maltose transporters inhibit Salmonella enterica growth and virulence

Neha Jain; Subodh Kumar Mishra; Uma Shankar; Arpita Tawani; Ankit Jaiswal; Tarun Kumar Sharma; Prashant Kodgire; Amit Kumar

Templates and Primers were procured from Sigma-Aldrich Chemicals Ltd. (St. Louis, MO, USA) ( Table S5a ). The reaction was performed in the master mix consisting of 1X PCR buffer, 0.33 mM dNTPs, 2 μM control culture DMSO was added as 9-amino acridine was dissolved in it. All the flasks were kept at 37˚C, 220 rpm for 45 min. Subsequently, samples were centrifuged at 12000 rpm and immediately preceded for RNA isolation. S. enterica culture prior to RNA isolation was treated with RNA protect reagent (Qiagen, USA) to stabilize RNA and prevent it from degradation. Treated and untreated Salmonella cultures were used for total RNA isolation. RNA isolation for all samples was carried out using TRIZOL reagent (Invitrogen) according to manufacturer's instructions. After RNA isolation, its concentration and purity was measured using NanoDrop (Thermoscientific) as ng/ul and A260/A280, respectively. Subsequently, all the RNA samples were treated with RNase-free DNaseI (Invitrogen) as mentioned by the manufacturer. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/357046 doi: bioRxiv preprint