Author: Sarah Sierra Smaga; Chaoyi Xu; Brady James Summers; Katherine Marie Digianantonio; Juan Roberto Perilla; Yong Xiong
Title: MxB restricts HIV-1 by targeting the tri-hexamer interface of the viral capsid Document date: 2018_10_15
ID: jdoax8co_8
Snippet: 175 Tri-hexamer interface mutations that escape MxB restriction also abolish MxB 1-35 -MBPdi 176 binding 177 Three mutations at the tri-hexamer interface (P207S, G208R and T210K) ( Fig 4A) were 178 previously identified in an in vitro evolution experiment to escape MxB restriction to varying 179 degrees [9] . The mutation P207S is relatively conservative, while both G208R and T210K 180 introduce bulky, positively charged residues to the predomina.....
Document: 175 Tri-hexamer interface mutations that escape MxB restriction also abolish MxB 1-35 -MBPdi 176 binding 177 Three mutations at the tri-hexamer interface (P207S, G208R and T210K) ( Fig 4A) were 178 previously identified in an in vitro evolution experiment to escape MxB restriction to varying 179 degrees [9] . The mutation P207S is relatively conservative, while both G208R and T210K 180 introduce bulky, positively charged residues to the predominantly negatively charged 181 environment described above (Fig 2A, 3A ). Based on our interaction mapping results, we 182 predicted that both G208R and T210K, which drastically alter the electrostatic environment of the 183 tri-hexamer interface, would decrease MxB binding to capsid. To test this hypothesis, we 184 introduced these mutations into CA tubes and performed co-pelleting assays with MxB 1-35 -185 MBPdi.
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