Author: Wang, Lingshu; Shi, Wei; Joyce, M. Gordon; Modjarrad, Kayvon; Zhang, Yi; Leung, Kwanyee; Lees, Christopher R.; Zhou, Tongqing; Yassine, Hadi M.; Kanekiyo, Masaru; Yang, Zhi-yong; Chen, Xuejun; Becker, Michelle M.; Freeman, Megan; Vogel, Leatrice; Johnson, Joshua C.; Olinger, Gene; Todd, John P.; Bagci, Ulas; Solomon, Jeffrey; Mollura, Daniel J.; Hensley, Lisa; Jahrling, Peter; Denison, Mark R.; Rao, Srinivas S.; Subbarao, Kanta; Kwong, Peter D.; Mascola, John R.; Kong, Wing-Pui; Graham, Barney S.
Title: Evaluation of candidate vaccine approaches for MERS-CoV Cord-id: l6wqi58t Document date: 2015_7_28
ID: l6wqi58t
Snippet: The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) as a cause of severe respiratory disease highlights the need for effective approaches to CoV vaccine development. Efforts focused solely on the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein may not optimize neutralizing antibody (NAb) responses. Here we show that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS-CoV strains in mice
Document: The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) as a cause of severe respiratory disease highlights the need for effective approaches to CoV vaccine development. Efforts focused solely on the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein may not optimize neutralizing antibody (NAb) responses. Here we show that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS-CoV strains in mice and non-human primates. Serological analysis and isolation of murine monoclonal antibodies revealed that immunization elicits NAbs to RBD and, non-RBD portions of S1 and S2 subunit. Multiple neutralization mechanisms were demonstrated by solving the atomic structure of a NAb-RBD complex, through sequencing of neutralization escape viruses and by constructing MERS-CoV S variants for serological assays. Immunization of rhesus macaques confers protection against MERS-CoV-induced radiographic pneumonia, as assessed using computerized tomography, supporting this strategy as a promising approach for MERS-CoV vaccine development.
Search related documents:
Co phrase search for related documents- absence presence and adenoviral vector: 1
- absence presence and live virus: 1, 2
- absence presence and low production: 1
- absence presence and luciferase activity: 1, 2, 3, 4, 5
- absence presence and lung capacity: 1
- absence presence and lung disease: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- absence presence and lung pathology: 1
- absence presence and lung segmentation: 1, 2
- absence presence and lung volume: 1
- absence presence and mab absence presence: 1
- acute respiratory infection and additional improvement: 1
- acute respiratory infection and live virus: 1, 2, 3, 4, 5, 6, 7, 8, 9
- acute respiratory infection and low production: 1, 2, 3
- acute respiratory infection and lung capacity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- acute respiratory infection and lung disease: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- acute respiratory infection and lung infiltrate: 1, 2, 3
- acute respiratory infection and lung pathology: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- acute respiratory infection and lung region: 1
- acute respiratory infection and lung volume: 1, 2, 3, 4, 5, 6
Co phrase search for related documents, hyperlinks ordered by date