Author: Norio Yamamoto; Shutoku Matsuyama; Tyuji Hoshino; Naoki Yamamoto
Title: Nelfinavir inhibits replication of severe acute respiratory syndrome coronavirus 2 in vitro. Document date: 2020_4_8
ID: 662pfa61_9
Snippet: Notably, nelfinavir (EC 50 = 1.13 µM, CC 50 = 24.32 µM, SI = 21.52) potently inhibited virus replication at the lowest concentration and exhibited the highest SI in the tested HIV-1 protease inhibitors (Fig. 1a, j) . To examine the target step of HIV-1 protease inhibitors in the virus life cycle, time-of-addition assays were performed. Intra-cellular viral RNA was quantified at 6 hpi with varying timing of drug addition. Nelfinavir and lopinavi.....
Document: Notably, nelfinavir (EC 50 = 1.13 µM, CC 50 = 24.32 µM, SI = 21.52) potently inhibited virus replication at the lowest concentration and exhibited the highest SI in the tested HIV-1 protease inhibitors (Fig. 1a, j) . To examine the target step of HIV-1 protease inhibitors in the virus life cycle, time-of-addition assays were performed. Intra-cellular viral RNA was quantified at 6 hpi with varying timing of drug addition. Nelfinavir and lopinavir suppressed multiplication of viral RNA in the cells even when these drugs were added at 3.5 hpi (Fig. S1 ). These results suggest that both drugs functioned at the post-entry step.
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