Prediction and Evolution of B Cell Epitopes of Surface Protein in SARS-CoV-2

Jerome R Lon; Yunmeng Bai; Bingxu Zhong; Fuqiang Cai; Hongli Du

The higher the conservation score calculated by the Consurf Server is, the more likely the site is to be mutated in the evolutionary process. When the score<1, the site is likely to be a conservative site; when the score is between 1 and 2, the site is a site which is likely to be a relatively easy mutation; when the score>2, the site is likely to be an easy mutation site [14] . In the 7 epitopes obtained, all the epitopes of the S, E, M protein were absolute conservative among all SARS-CoV-2 sequences. For the human coronavirus dataset and the coronavirus dataset, only the average conservative score of the epitope D is higher than 1, which is prone to mutation. The epitope D should not be used as an epitope of the S protein. The conservation of the epitope H could not be calculated by the PDB file, the application value of the epitope H needed further experimental verification. Although the epitopes could be integrally considered to be conservative, the independent residues of these epitopes could still easy to mutate.