Author: Danielle E Anderson; Jin Cui; Qian Ye; Baoying Huang; Wenhong Zu; Jing Gong; Weiqiang Liu; So Young Kim; Biao Guo Yan; Kristmundur Sigmundsson; Xiao Fang Lim; Fei Ye; Peihua Niu; Xuming Zhou; Wenjie Tan; Lin-Fa Wang; Xu Tan
Title: Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy Document date: 2020_3_30
ID: 0l33i6s4_12
Snippet: The other 34 genes have 1-3 sgRNAs due to the constraints of the gene sequences for designing 6 sgRNAs. Selection of sgRNAs was based on the calculations of off-target scores to lower potential 7 off-target effects of the sgRNAs. The sgRNAs designs were validated for 21 sgRNAs targeting 11 8 genes using T7E1 endonuclease cleavage assay or Western blotting. We observed 90.5% of 9 effectiveness in gene editing or gene knockdown, supporting the qual.....
Document: The other 34 genes have 1-3 sgRNAs due to the constraints of the gene sequences for designing 6 sgRNAs. Selection of sgRNAs was based on the calculations of off-target scores to lower potential 7 off-target effects of the sgRNAs. The sgRNAs designs were validated for 21 sgRNAs targeting 11 8 genes using T7E1 endonuclease cleavage assay or Western blotting. We observed 90.5% of 9 effectiveness in gene editing or gene knockdown, supporting the quality of the designed library 10 (Supplementary Figure 1) . 11
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