Selected article for: "genomic dna and reference strain"
Author: Mélanie Legrand; Sophie Bachellier-Bassi; Keunsook K. Lee; Yogesh Chaudhari; Hélène Tournu; Laurence Arbogast; Hélène Boyer; Murielle Chauvel; Vitor Cabral; Corinne Maufrais; Audrey Nesseir; Irena Maslanka; Emmanuelle Permal; Tristan Rossignol; Louise A. Walker; Ute Zeidler; Sadri Znaidi; Floris Schoeters; Charlotte Majgier; Renaud A. Julien; Laurence Ma; Magali Tichit; Christiane Bouchier; Patrick Van Dijck; Carol A. Munro; Christophe d’Enfert
Title: Generating genomic platforms to study Candida albicans pathogenesis
  • Document date: 2018_2_8
    • ID: 1vx62ofn_76
    Snippet: In eukaryotes, ORFeomes are usually cloned from full-length cDNA libraries to account for the presence of introns and therefore splicing variants. However, only 6% of C. albicans genes have introns (55) , rendering the generation of the C. albicans ORFeome more straightforward than for pluricellular eukaryotes. Indeed, ORFs were simply amplified from the genomic DNA of the reference strain SC5314 and therefore, 360 ORFs in the C. albicans.....
    Document: In eukaryotes, ORFeomes are usually cloned from full-length cDNA libraries to account for the presence of introns and therefore splicing variants. However, only 6% of C. albicans genes have introns (55) , rendering the generation of the C. albicans ORFeome more straightforward than for pluricellular eukaryotes. Indeed, ORFs were simply amplified from the genomic DNA of the reference strain SC5314 and therefore, 360 ORFs in the C. albicans

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