Author: Danielle E Anderson; Jin Cui; Qian Ye; Baoying Huang; Wenhong Zu; Jing Gong; Weiqiang Liu; So Young Kim; Biao Guo Yan; Kristmundur Sigmundsson; Xiao Fang Lim; Fei Ye; Peihua Niu; Xuming Zhou; Wenjie Tan; Lin-Fa Wang; Xu Tan
Title: Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy Document date: 2020_3_30
ID: 0l33i6s4_31
Snippet: shown by both immunofluorescence staining and virus tittering assay (Fig. 3c, 3d and 3e) . In 5 contrast, supplementation of methyl-group donor or thymidine had little effect on viral replication, 6 evidence that it is the purine synthesis activity of MTHFD1 that is essential for the viral replication 7 (Fig. 3d) . To further pinpoint the enzymatic function of MTHFD1 in viral replication, we 8 performed gene complementation experiments in an shRN.....
Document: shown by both immunofluorescence staining and virus tittering assay (Fig. 3c, 3d and 3e) . In 5 contrast, supplementation of methyl-group donor or thymidine had little effect on viral replication, 6 evidence that it is the purine synthesis activity of MTHFD1 that is essential for the viral replication 7 (Fig. 3d) . To further pinpoint the enzymatic function of MTHFD1 in viral replication, we 8 performed gene complementation experiments in an shRNA cell line with over 90% knockdown 9 of MTHFD1 (Fig. 2i) . The block of Zika virus replication in this cell line was completely rescued 10 by overexpression of an shRNA-resistant MTHFD1 construct or bat homolog ( Fig. 3f and 3g) . 11
Search related documents:
Co phrase search for related documents- cell line and immunofluorescence staining: 1, 2, 3, 4, 5, 6, 7
- cell line and little effect: 1, 2
- immunofluorescence staining and methyl group: 1
- MTHFD1 construct and shrna resistant MTHFD1 construct: 1
- MTHFD1 synthesis activity and purine MTHFD1 synthesis activity: 1
Co phrase search for related documents, hyperlinks ordered by date