Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy

Danielle E Anderson; Jin Cui; Qian Ye; Baoying Huang; Wenhong Zu; Jing Gong; Weiqiang Liu; So Young Kim; Biao Guo Yan; Kristmundur Sigmundsson; Xiao Fang Lim; Fei Ye; Peihua Niu; Xuming Zhou; Wenjie Tan; Lin-Fa Wang; Xu Tan

DEseq2. The log2 ratio of counts between conditions were calculated. The log2 fold change of 28 reads count of infected samples to mock infected samples were then used as the input for RIGER 29 algorithm as previously described (RNAi Gene Enrichment Ranking, GENE-E) 1 . The log-fold-30 change metric for ranking sgRNAs and the weighted sum method (weighted sum of first two ranks 31 of sgRNAs for a gene) with 1 × 10 6 permutations were used to convert individual sgRNA to genes. 32 . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020. 03.29.014209 doi: bioRxiv preprint In the output of RIGER analysis, a given gene was assigned a normalized enrichment score (NES) 1 to give the ranked gene list. Hits were defined as those owning p-value smaller than 0.001. 2 3 siRNA screen 4