Selected article for: "HIV replication and host cell"
Author: Selberg, Simona; Žusinaite, Eva; Herodes, Koit; Seli, Neinar; Kankuri, Esko; Merits, Andres; Karelson, Mati
Title: HIV Replication Is Increased by RNA Methylation METTL3/METTL14/WTAP Complex Activators
  • Cord-id: ovpxx32z
  • Document date: 2021_6_11
    • ID: ovpxx32z
    Snippet: [Image: see text] The N6-methyladenosine (m(6)A) modifications in both viral and host cell RNAs play an important role in HIV-1 virus genome transcription and virus replication. We demonstrate here that activators of the METTL3/METTL14/WTAP RNA methyltransferase complex enhance the production of virus particles in cells harboring HIV-1 provirus. In parallel, the amount of m(6)A residues in the host cell mRNA was increased in the presence of these activator compounds. Importantly, the m(6)A methy
    Document: [Image: see text] The N6-methyladenosine (m(6)A) modifications in both viral and host cell RNAs play an important role in HIV-1 virus genome transcription and virus replication. We demonstrate here that activators of the METTL3/METTL14/WTAP RNA methyltransferase complex enhance the production of virus particles in cells harboring HIV-1 provirus. In parallel, the amount of m(6)A residues in the host cell mRNA was increased in the presence of these activator compounds. Importantly, the m(6)A methylation of the HIV-1 RNA was also enhanced significantly (about 18%). The increase of virus replication by the small-molecule activators of the METTL3/METTL14/WTAP complex excludes them as potential anti-HIV-1 drug candidates. However, the compounds may be of large interest as activators for the latent HIV-1 provirus copies deposited in host cells’ genome and the subsequent virus eradication by an antiviral compound.

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