Generating genomic platforms to study Candida albicans pathogenesis

Mélanie Legrand; Sophie Bachellier-Bassi; Keunsook K. Lee; Yogesh Chaudhari; Hélène Tournu; Laurence Arbogast; Hélène Boyer; Murielle Chauvel; Vitor Cabral; Corinne Maufrais; Audrey Nesseir; Irena Maslanka; Emmanuelle Permal; Tristan Rossignol; Louise A. Walker; Ute Zeidler; Sadri Znaidi; Floris Schoeters; Charlotte Majgier; Renaud A. Julien; Laurence Ma; Magali Tichit; Christiane Bouchier; Patrick Van Dijck; Carol A. Munro; Christophe d’Enfert

However, many challenges remain to be addressed. For heterozygous genes, only one allele is present in the ORFeome. Because functional differences have been reported between the 2 alleles of a heterozygous gene (57, 58) , it would be relevant to also clone and validate the second allele in these instances. In addition, oligonucleotides have been designed based on annotation of the haploid set of Assembly 19 of the C. albicans genome. As a consequence, genes of the MTLα locus have not been included in the ORFeome. Despite our efforts, the collection is still missing 1,081 clones. The next version of the C. albicans ORFeome could author/funder. All rights reserved. No reuse allowed without permission.