Pangolin homology associated with 2019-nCoV

Tao Zhang; Qunfu Wu; Zhigang Zhang

Data preparation 2 We downloaded raw data of lung08 and lung07 published by Liu's study (7) Read Assembly and construction of consensus sequence 10 Virus-targeted reads were assembled de novo using MEGAHIT (v1.1.3)(12). Read 11 remapping to assembled contigs was performed by using Bowtie2(11). Mapping coverage 12 and depth were produced using Samtools (version 1.9)(13). Contigs were taxonomically 13 annotated using BLAST 2.9.0+ against 2845 Coronavirus reference genomes (Table S1) Phylogenetic relationship analysis 9 Sequence alignment was carried out using MUSCLE software(14). Alignment 10 accuracy was checked manually base-by-base. Gblocks (15) The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.19.950253 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.19.950253 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.19.950253 doi: bioRxiv preprint Table S2 .