Author: Beltrán-Pavez, Carolina; Márquez, Chantal L.; Muñoz, Gabriela; Valiente-EcheverrÃa, Fernando; Gaggero, Aldo; Soto-Rifo, Ricardo; Barriga, Gonzalo P.
Title: SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction Cord-id: pg6893i5 Document date: 2020_3_30
ID: pg6893i5
Snippet: The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis and considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR kits were compatible with direct SARS-CoV-2 detection from nasopharyngeal swab samples. We show that one of the tested kits is fully compatible with direct SARS-CoV-2 detection suggesting that omission of an RNA extraction step should be consider
Document: The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis and considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR kits were compatible with direct SARS-CoV-2 detection from nasopharyngeal swab samples. We show that one of the tested kits is fully compatible with direct SARS-CoV-2 detection suggesting that omission of an RNA extraction step should be considered in SARS-CoV-2 diagnosis.
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