Potential host range of multiple SARS-like coronaviruses and an improved ACE2-Fc variant that is potent against both SARS-CoV-2 and SARS-CoV-1

Yujun Li; Haimin Wang; Xiaojuan Tang; Danting Ma; Chengzhi Du; Yifei Wang; Hong Pan; Qing Zou; Jie Zheng; Liangde Xu; Michael Farzan; Guocai Zhong

Statistical analysis. Data expressed as mean values ± s.d. or s.e.m. Statistical analyses were performed using two-sided two-sample Student's t-test using GraphPad Prism 6.0 software when applicable. Differences were considered significant at P < 0.01. Data availability. Our research resources, including methods, plasmids, and protocols, are available upon reasonable request to qualified academic investigators for noncommercial research purposes. All reagents developed, such as vector plasmids, as well as detailed methods, will be made available upon written request. Figure 2a . b, SARS-CoV-2 RBD in complex with human ACE2. The residues absent in donkey ACE2, as shown in Extended Data Figure 1 , are indicated as magenta. c, Gating strategy for the flow cytometry data shown in Figures 2a and b . The left panel shows the preliminary FSC/SSC gate of the starting cell population. The right panel shows the gates that defines "negative" (red) and "positive" (purple) staining cell populations, respectively.