Selected article for: "reference method and RT PCR method"
Author: Schnuriger, Aurélie; Perrier, Marine; Marinho, Valérie; Michel, Yanne; Saloum, Kenda; Boukli, Narjis; Lambert-Niclot, Sidonie; Amiel, Corinne; Fofana, Djeneba Bocar; Gozlan, Joël; Morand-Joubert, Laurence
Title: Caution in interpretation of SARS-CoV-2 quantification based on RT-PCR cycle threshold value
  • Cord-id: m4k8cqg7
  • Document date: 2021_3_4
    • ID: m4k8cqg7
    Snippet: RT-PCR is the reference method for diagnosis of a Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection. During the setting up of 6 SARS-CoV-2 RT-PCR assays in our laboratory, comparative evaluations were systematically undertaken and allowed to evidence major discrepancies on cycle threshold RT-PCR results between techniques. These tendencies were confirmed in routine application when analyzing sequential samples from the same patients. Our aim was to examine the impact of the
    Document: RT-PCR is the reference method for diagnosis of a Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection. During the setting up of 6 SARS-CoV-2 RT-PCR assays in our laboratory, comparative evaluations were systematically undertaken and allowed to evidence major discrepancies on cycle threshold RT-PCR results between techniques. These tendencies were confirmed in routine application when analyzing sequential samples from the same patients. Our aim was to examine the impact of the technique among factors influencing RT-PCR result, a far surrogate of ‘viral load’ in the heterogeneous environment of respiratory specimens.

    Search related documents:
    Co phrase search for related documents
    • abbott sars assay and acute respiratory sars syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
    • abbott sars assay and low estimate: 1
    • acid testing and acute infection diagnosis: 1, 2
    • acid testing and acute respiratory: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • acid testing and acute respiratory sars syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • acid testing and longitudinal monitoring: 1
    • acute respiratory and local equipment: 1, 2, 3, 4, 5
    • acute respiratory and local equipment availability: 1
    • acute respiratory and longitudinal monitoring: 1, 2, 3, 4, 5, 6
    • acute respiratory and longitudinal sample: 1, 2, 3, 4, 5, 6
    • acute respiratory and low estimate: 1, 2, 3, 4, 5, 6
    • acute respiratory sars syndrome and local equipment: 1, 2, 3
    • acute respiratory sars syndrome and longitudinal monitoring: 1, 2
    • acute respiratory sars syndrome and longitudinal sample: 1, 2
    • acute respiratory sars syndrome and low estimate: 1, 2, 3, 4, 5, 6