Author: Junan Zhu; Kristina Rivera; Dror Baron
Title: Noisy Pooled PCR for Virus Testing Document date: 2020_4_8
ID: 8kccpd4x_5
Snippet: Recently, researchers across the world have been looking to increase the sample size per PCR run by using custom barcodes for each sample and then pooling them together [5] . Custom barcoding is not new in terms of multiplexed genetic sequencing [6] . Briefly, custom barcodes for each patients RNA sample are designed by an algorithm and substituted in as the reverse transcriptase (RT) primers to generate barcoded cDNA. Next-generation sequencing .....
Document: Recently, researchers across the world have been looking to increase the sample size per PCR run by using custom barcodes for each sample and then pooling them together [5] . Custom barcoding is not new in terms of multiplexed genetic sequencing [6] . Briefly, custom barcodes for each patients RNA sample are designed by an algorithm and substituted in as the reverse transcriptase (RT) primers to generate barcoded cDNA. Next-generation sequencing is performed after a single pooled PCR reaction, and then demultiplexed to determine each samples viral content. Our method of pooling samples before adding barcodes could be used by researchers for a quicker time to analysis and also as a complementary method to reanalyze barcoded samples.
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