Author: Cara E. Brook; Mike Boots; Kartik Chandran; Andrew P. Dobson; Christian Drosten; Andrea L. Graham; Bryan T. Grenfell; Marcel A. Müller; Melinda Ng; Lin-Fa Wang; Anieke van Leeuwen
Title: Accelerated viral dynamics in bat cell lines, with implications for zoonotic emergence Document date: 2019_7_8
ID: 683qcgd9_9
Snippet: Prep kit, according to the manufacturer's instructions and including the step for cellular DNA 525 digestion. Post-extraction, RNA quality was verified via nanodrop, and RNA was converted to 526 cDNA using the Invitrogen Superscript III cDNA synthesis kit, according to the manufacturer's 527 instructions. cDNA was then stored at 4ËšC and as a frozen stock at -20ËšC to await qPCR. 528.....
Document: Prep kit, according to the manufacturer's instructions and including the step for cellular DNA 525 digestion. Post-extraction, RNA quality was verified via nanodrop, and RNA was converted to 526 cDNA using the Invitrogen Superscript III cDNA synthesis kit, according to the manufacturer's 527 instructions. cDNA was then stored at 4ËšC and as a frozen stock at -20ËšC to await qPCR. 528
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