Selected article for: "negative sense and viral negative sense RNA synthesis"
Author: Myra Hosmillo; Jia Lu; Michael R. McAllaster; James B. Eaglesham; Xinjie Wang; Edward Emmott; Patricia Domingues; Yasmin Chaudhry; Timothy J Fitzmaurice; Matthew K.H. Tung; Marc Panas; Gerald McInerney; Nicholas Locker; Craig B. Willen; Ian Goodfellow
Title: Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation
  • Document date: 2019_3_8
    • ID: d0q5lhf4_25
    Snippet: Similar results were obtained following transfection of viral RNA into cells to bypass 286 the entry phase; viral positive and negative sense RNA synthesis was near (or 287 below) the sensitivity of the assay following transfection of viral VPg-linked RNA into 288 two independent ΔG3BP1 cell lines ( presence of low levels of viral infectivity ( Fig 7B) . This discrepancy likely reflects the 293 relative sensitivities of the assays and the nature.....
    Document: Similar results were obtained following transfection of viral RNA into cells to bypass 286 the entry phase; viral positive and negative sense RNA synthesis was near (or 287 below) the sensitivity of the assay following transfection of viral VPg-linked RNA into 288 two independent ΔG3BP1 cell lines ( presence of low levels of viral infectivity ( Fig 7B) . This discrepancy likely reflects the 293 relative sensitivities of the assays and the nature of the strand specific qPCR assay 294 which requires low levels of RNA input to maintain strand specificity. Together these 295 data suggest that the function of G3BP1 is prior to, or at the level of viral negative 296 sense RNA synthesis, with the most logical steps being either viral RNA translation 297 or the formation of viral replication complexes. 298 299 G3BP1 is required for the association of VPg with 40S ribosomal subunits. 300

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