Metformin attenuates silica-induced pulmonary fibrosis via AMPK signaling

Cheng, Demin; Xu, Qi; Wang, Yue; Li, Guanru; Sun, Wenqing; Ma, Dongyu; Zhou, Siyun; Liu, Yi; Han, Lei; Ni, Chunhui

BACKGROUND: Silicosis is one of the most common occupational pulmonary fibrosis caused by respirable silica-based particle exposure, with no ideal drugs at present. Metformin, a commonly used biguanide antidiabetic agent, could activate AMP-activated protein kinase (AMPK) to exert its pharmacological action. Therefore, we sought to investigate the role of metformin in silica-induced lung fibrosis. METHODS: The anti-fibrotic role of metformin was assessed in 50 mg/kg silica-induced lung fibrosis model. Silicon dioxide (SiO(2))-stimulated lung epithelial cells/macrophages and transforming growth factor-beta 1 (TGF-β1)-induced differentiated lung fibroblasts were used for in vitro models. RESULTS: At the concentration of 300 mg/kg in the mouse model, metformin significantly reduced lung inflammation and fibrosis in SiO(2)-instilled mice at the early and late fibrotic stages. Besides, metformin (range 2–10 mM) reversed SiO(2)-induced cell toxicity, oxidative stress, and epithelial-mesenchymal transition process in epithelial cells (A549 and HBE), inhibited inflammation response in macrophages (THP-1), and alleviated TGF-β1-stimulated fibroblast activation in lung fibroblasts (MRC-5) via an AMPK-dependent pathway. CONCLUSIONS: In this study, we identified that metformin might be a potential drug for silicosis treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-021-03036-5.