Selected article for: "agarose gel and Assay kit"
Author: Yueting Zhang; Gary Whittaker
Title: Productive infection of field strains of avian coronavirus infectious bronchitis virus in chicken peripheral blood-derived monocyte
  • Document date: 2016_2_26
    • ID: lcpp5fim_6
    Snippet: Step RT--PCR kit (Invitrogen). IBV N gene primers were previously described by (17). For apoptosis assays, chPBMCs were infected with 2x10 3 TCID50 IBV--M41 and at 6h post infection, we used a Green FLICA caspase 3/7 assay kit (ImmunoChemistry Technologies). For the DNA laddering assay, total DNA was extracted from chPBMCs using a Qiagen DNeasy Blood & Tissue kit, and laddering was visualized on an agarose gel. To inactivate IBV--M41, viruses wer.....
    Document: Step RT--PCR kit (Invitrogen). IBV N gene primers were previously described by (17). For apoptosis assays, chPBMCs were infected with 2x10 3 TCID50 IBV--M41 and at 6h post infection, we used a Green FLICA caspase 3/7 assay kit (ImmunoChemistry Technologies). For the DNA laddering assay, total DNA was extracted from chPBMCs using a Qiagen DNeasy Blood & Tissue kit, and laddering was visualized on an agarose gel. To inactivate IBV--M41, viruses were irradiated for 30min under a UV--lamp (wavelength 254nm).

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