Author: Steven Henikoff; Jorja G. Henikoff
Title: Profiling the epigenome at home Document date: 2020_4_17
ID: mojf4l6b_18
Snippet: • Resuspend and withdraw enough of the ConA bead slurry such that there will be 3-5 μL for each final sample of up to ~100,000 mammalian cells. The following is for 16 samples. • Transfer 85 μL ConA bead slurry into 1 mL Binding buffer in a 1.5 mL tube and mix by pipetting. Place the tube on a magnet stand to clear (30 s to 2 min). • Withdraw the liquid completely, and remove from the magnet stand. Add 1 mL Binding buffer and mix by pipet.....
Document: • Resuspend and withdraw enough of the ConA bead slurry such that there will be 3-5 μL for each final sample of up to ~100,000 mammalian cells. The following is for 16 samples. • Transfer 85 μL ConA bead slurry into 1 mL Binding buffer in a 1.5 mL tube and mix by pipetting. Place the tube on a magnet stand to clear (30 s to 2 min). • Withdraw the liquid completely, and remove from the magnet stand. Add 1 mL Binding buffer and mix by pipetting. • Place on magnet stand to clear, withdraw liquid, and resuspend in 85 μL Binding buffer (for 5 μL per sample).
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