Selected article for: "fusion activity and glycoprotein fusion"
Author: Changhai Lei; Wenyan Fu; Kewen Qian; Tian Li; Sheng Zhang; Min Ding; Shi Hu
Title: Potent neutralization of 2019 novel coronavirus by recombinant ACE2-Ig
  • Document date: 2020_2_2
    • ID: aeuy92bx_19
    Snippet: A quantitative cell fusion assay based on β -galactosidase (β-gal) as a reporter gene was used for the assessment of the neutralization activity of the fusion proteins, as described previously 26 . 293T cells transfected with the indicated CoV S glycoprotein genes were preincubated with different fusion proteins at room temperature for 15 min, then mixed with 293T cells transfected with ACE2 at 1:1 ratio and incubated at 37°C for 4 h. Cells we.....
    Document: A quantitative cell fusion assay based on β -galactosidase (β-gal) as a reporter gene was used for the assessment of the neutralization activity of the fusion proteins, as described previously 26 . 293T cells transfected with the indicated CoV S glycoprotein genes were preincubated with different fusion proteins at room temperature for 15 min, then mixed with 293T cells transfected with ACE2 at 1:1 ratio and incubated at 37°C for 4 h. Cells were then lysed, and the β -gal activity was measured. The protein concentrations during fusion were used for calculation of the IC50 defined as the concentration at which the β -gal activity was reduced by 50%.

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