Author: Steven Henikoff; Jorja G. Henikoff
Title: Profiling the epigenome at home Document date: 2020_4_17
ID: mojf4l6b_28
Snippet: • Place tubes on the magnet stand to clear. Withdraw the liquid with the pipettor set to 5 µL less than the volume to be removed. • Mix the secondary antibody 1:100 in Wash buffer and squirt in 50 µL per sample while gently vortexing to allow the solution to dislodge the beads from the sides. Tip: Although not needed for CUT&RUN, the secondary antibody step is required for CUT&Tag to increase the number of Protein A binding sites for each b.....
Document: • Place tubes on the magnet stand to clear. Withdraw the liquid with the pipettor set to 5 µL less than the volume to be removed. • Mix the secondary antibody 1:100 in Wash buffer and squirt in 50 µL per sample while gently vortexing to allow the solution to dislodge the beads from the sides. Tip: Although not needed for CUT&RUN, the secondary antibody step is required for CUT&Tag to increase the number of Protein A binding sites for each bound antibody. We have found that without the secondary antibody the efficiency is very low. • Place the tubes on a Rotator and rotate at room temperature for 30 min.
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