Author: Jingyue Ju; Xiaoxu Li; Shiv Kumar; Steffen Jockusch; Minchen Chien; Chuanjuan Tao; Irina Morozova; Sergey Kalachikov; Robert N. Kirchdoerfer; James J. Russo
Title: Nucleotide Analogues as Inhibitors of SARS-CoV Polymerase Document date: 2020_3_14
ID: hj675z1b_7
Snippet: The sequence of the primer and template used for these extension reactions is shown at the top of the figure. Polymerase extension reactions were performed by incubating the primer and template with 2'-F,Me-UTP and the appropriate reaction buffer for the specific enzyme, followed by detection of the reaction products by MALDI-TOF MS. The MS spectra for Therminator II (T2) in (a) and for Therminator IX (T9) in (b) indicates single-base incorporati.....
Document: The sequence of the primer and template used for these extension reactions is shown at the top of the figure. Polymerase extension reactions were performed by incubating the primer and template with 2'-F,Me-UTP and the appropriate reaction buffer for the specific enzyme, followed by detection of the reaction products by MALDI-TOF MS. The MS spectra for Therminator II (T2) in (a) and for Therminator IX (T9) in (b) indicates single-base incorporation and termination, while the MS spectrum for Thermo Sequenase (TS) in (c) indicates no incorporation, showing only a primer peak. The accuracy for m/z determination is ± 10 Da.
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