Author: Shi, Z.; Dong, N.; Lai, X.; Yu, H.; Li, D.
Title: Centrifugal Step Emulsification Microfluidics Supporting Droplet Digital Loop-Mediated Isothermal Amplification (LAMP) of Sars-Cov-2 N Gene Cord-id: k5zcnas2 Document date: 2021_1_1
ID: k5zcnas2
Snippet: Responding to the global crisis of SARS-CoV-2 (COVID-19), the centrifugal step emulsification microfluidics supporting droplet digital Loop-mediated isothermal amplification (LAMP) is presented to quantify the SARS-COV-2 N Gene in this study. The designed centrifugal step emulsification chip with a triangular nozzle shows a robust droplet generation for sample division for LAMP, and the influence by channel flow resistance is experimentally analyzed. In addition, an evaporation-resisted structur
Document: Responding to the global crisis of SARS-CoV-2 (COVID-19), the centrifugal step emulsification microfluidics supporting droplet digital Loop-mediated isothermal amplification (LAMP) is presented to quantify the SARS-COV-2 N Gene in this study. The designed centrifugal step emulsification chip with a triangular nozzle shows a robust droplet generation for sample division for LAMP, and the influence by channel flow resistance is experimentally analyzed. In addition, an evaporation-resisted structure is applied to address the reagent loss in the heating process caused by PDMS material. The devices and solution are potential to be a promising tool for the quantification of SARS-CoV-2. © 2021 IEEE.
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