Author: Cara E. Brook; Mike Boots; Kartik Chandran; Andrew P. Dobson; Christian Drosten; Andrea L. Graham; Bryan T. Grenfell; Marcel A. Müller; Melinda Ng; Lin-Fa Wang; Anieke van Leeuwen
Title: Accelerated viral dynamics in bat cell lines, with implications for zoonotic emergence Document date: 2019_7_8
ID: 683qcgd9_8
Snippet: For these gene expression time series, four 6-well plates for each cell line-virus 518 combination were incubated with virus for one hour at 37ËšC. Following incubation, virus was 519 aspirated off, and cell monolayers were washed in PBS, then covered with an agar plaque assay 520 overlay to mimic conditions under which infection trials were run. Plates were then harvested 521 sequentially at timepoints of roughly 5, 10, 15, and 20 hours post-inf.....
Document: For these gene expression time series, four 6-well plates for each cell line-virus 518 combination were incubated with virus for one hour at 37ËšC. Following incubation, virus was 519 aspirated off, and cell monolayers were washed in PBS, then covered with an agar plaque assay 520 overlay to mimic conditions under which infection trials were run. Plates were then harvested 521 sequentially at timepoints of roughly 5, 10, 15, and 20 hours post-infection (exact timing varied 522 as multiple trials were running simultaneously). Upon harvest of each plate, agar overlay was 523 removed, and virus was lysed and RNA extracted from cells using the Zymo Quick RNA Mini 524
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