Selected article for: "cell line and copy cell"
Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells
  • Document date: 2018_12_26
    • ID: 1kugu5zk_30
    Snippet: In the first library, we combined one of three localization tags (NLS (nucleus), NES (cytoplasm) and Lyn (plasma membrane)) with four fluorescent proteins (tagBFP, mAzamiGreen, mScarlet and iRFP713) (Fig. 5a) . Performed in parallel, this reaction generated The final library was delivered to the hAAVS1-LP HEK293T cell line described before (Fig. 4 ) to ensure single-copy integration in every cell. We visualized the expression and localization of .....
    Document: In the first library, we combined one of three localization tags (NLS (nucleus), NES (cytoplasm) and Lyn (plasma membrane)) with four fluorescent proteins (tagBFP, mAzamiGreen, mScarlet and iRFP713) (Fig. 5a) . Performed in parallel, this reaction generated The final library was delivered to the hAAVS1-LP HEK293T cell line described before (Fig. 4 ) to ensure single-copy integration in every cell. We visualized the expression and localization of the fluorescent proteins after selection with Blasticidin and observed the successful random delivery of no more than 3 fluorescently-tagged subcellular compartments (Fig. 5b) , as predicted from the cloning strategy.

    Search related documents:
    Co phrase search for related documents
    • Blasticidin selection and fluorescent protein: 1
    • cell line and clone strategy: 1
    • cell line and fluorescent protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cell line and localization expression: 1, 2, 3, 4
    • cell line and parallel perform: 1
    • cell line and plasma membrane: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cell line and single copy: 1, 2, 3
    • clone strategy and fluorescent protein: 1
    • fluorescent protein and localization expression: 1, 2, 3, 4, 5, 6
    • fluorescent protein and localization tag: 1
    • fluorescent protein and NES cytoplasm: 1
    • fluorescent protein and plasma membrane: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • fluorescent protein localization expression and localization expression: 1, 2
    • localization expression and NES cytoplasm: 1
    • localization expression and plasma membrane: 1, 2, 3, 4, 5