Selected article for: "negative sense and positive sense"
Author: Telwatte, Sushama; Kumar, Nitasha; Vallejo-Gracia, Albert; Kumar, G. Renuka; Lu, Chuanyi M.; Ott, Melanie; Wong, Joseph K.; Yukl, Steven A.
Title: Novel RT-ddPCR Assays for determining the transcriptional profile of SARS-CoV-2
  • Cord-id: rs987d1w
  • Document date: 2021_1_12
    • ID: rs987d1w
    Snippet: The exact mechanism of coronavirus replication and transcription is not fully understood; however, a hallmark of coronavirus transcription is the generation of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and an array of subgenomic mRNAs (sgRNAs) encompassing sequences arising from discontinuous transcription. Existing PCR-based diagnostic assays for SAR-CoV-2 are qualitative or semi-quantitative and do not provide the resolu
    Document: The exact mechanism of coronavirus replication and transcription is not fully understood; however, a hallmark of coronavirus transcription is the generation of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and an array of subgenomic mRNAs (sgRNAs) encompassing sequences arising from discontinuous transcription. Existing PCR-based diagnostic assays for SAR-CoV-2 are qualitative or semi-quantitative and do not provide the resolution needed to assess the complex transcription dynamics of SARS-CoV-2 over the course of infection. We developed and validated a novel panel of specially designed SARS-CoV-2 ddPCR-based assays to map the viral transcription profile. Application of these assays to clinically relevant samples will enhance our understanding of SARS-CoV-2 replication and transcription and may also inform the development of improved diagnostic tools and therapeutics.

    Search related documents:
    Co phrase search for related documents