Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells Document date: 2018_12_26
ID: 1kugu5zk_44
Snippet: Immunofluorescence analysis with VP35 and 2A antibodies show characteristic cytoplasmic punctate structures (Fig. 7f) representing inclusion bodies that are sites of virus replication [28] [29] [30] , indicating correct localization of the P2A-tagged viral proteins in these stable cell lines. Due to the lack of available specific antibodies for the Lpol and the lack of a P2A tag in the construct, the Lpol expression cannot be assayed by Western b.....
Document: Immunofluorescence analysis with VP35 and 2A antibodies show characteristic cytoplasmic punctate structures (Fig. 7f) representing inclusion bodies that are sites of virus replication [28] [29] [30] , indicating correct localization of the P2A-tagged viral proteins in these stable cell lines. Due to the lack of available specific antibodies for the Lpol and the lack of a P2A tag in the construct, the Lpol expression cannot be assayed by Western blot or immunofluorescence analysis. However, the fact that these cells support minigenome activity ( Fig. 7b and 7d) confirms that Lpol is expressed. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/506188 doi: bioRxiv preprint
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