Author: Lifeng Zhou; Arun Richard Chandrasekaran; Jibin Abraham Punnoose; Gaston Bonenfant; Stephon Charles; Oksana Levchenko; Pheonah Badu; Cassandra Cavaliere; Cara T. Pager; Ken Halvorsen
Title: Programmable low-cost DNA-based platform for viral RNA detection Document date: 2020_1_16
ID: 8kced06y_27
Snippet: For the experiments of Fig. 1G and Fig. S8 , first, all nanoswitches were purified by LC and then their concentrations were determined by measuring A260 absorbance with a Thermo Scientific NanoDrop 2000. Nanoswitch mixtures were made by mixing nanoswitches in equimolar concentrations. The detection reaction volume is 10 µl with nanoswitch (0.1 nM), MgCl 2 (10mM), 1×PBS and blocking oligos (200 nM). The blocking oligos are short oligos (14 nucle.....
Document: For the experiments of Fig. 1G and Fig. S8 , first, all nanoswitches were purified by LC and then their concentrations were determined by measuring A260 absorbance with a Thermo Scientific NanoDrop 2000. Nanoswitch mixtures were made by mixing nanoswitches in equimolar concentrations. The detection reaction volume is 10 µl with nanoswitch (0.1 nM), MgCl 2 (10mM), 1×PBS and blocking oligos (200 nM). The blocking oligos are short oligos (14 nucleotides) that can prevent the binding of target RNA to the inner surface of plastic tubes. 27 The detection incubation was conducted in a thermal cycler with a thermal annealing from 40 °C to 25 °C for about 12 hours.
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