Author: Chenyu Li; David N. Debruyne; Julia Spencer; Vidushi Kapoor; Lily Y. Liu; Bo Zhou; Lucie Lee; Rounak Feigelman; Grayson Burdon; Jeffrey Liu; Alejandra Oliva; Adam Borcherding; Hongdong Tan; Alexander E. Urban; Guoying Liu; Zhitong Liu
Title: High sensitivity detection of coronavirus SARS-CoV-2 using multiplex PCR and a multiplex-PCR-based metagenomic method Document date: 2020_3_14
ID: 0hxan9rw_36
Snippet: Panel design is based on the SARS-CoV-2 sequence NC_045512.2 (https://www.ncbi.nlm.nih.gov/nuccore/NC_045512.2/). In total, 343 primer pairs, distributed into two pools, were selected by a proprietary panel design pipeline to cover the whole genome except for 92 bases at its ends. Primers were optimized to preferentially amplify the SARS-CoV-2 cDNA versus background human cDNA or genomic DNA. They were also optimized to uniformly amplify the cove.....
Document: Panel design is based on the SARS-CoV-2 sequence NC_045512.2 (https://www.ncbi.nlm.nih.gov/nuccore/NC_045512.2/). In total, 343 primer pairs, distributed into two pools, were selected by a proprietary panel design pipeline to cover the whole genome except for 92 bases at its ends. Primers were optimized to preferentially amplify the SARS-CoV-2 cDNA versus background human cDNA or genomic DNA. They were also optimized to uniformly amplify the covered genome.
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