Selected article for: "detection limit and genome coverage"

Author: Chenyu Li; David N. Debruyne; Julia Spencer; Vidushi Kapoor; Lily Y. Liu; Bo Zhou; Lucie Lee; Rounak Feigelman; Grayson Burdon; Jeffrey Liu; Alejandra Oliva; Adam Borcherding; Hongdong Tan; Alexander E. Urban; Guoying Liu; Zhitong Liu
Title: High sensitivity detection of coronavirus SARS-CoV-2 using multiplex PCR and a multiplex-PCR-based metagenomic method
  • Document date: 2020_3_14
  • ID: 0hxan9rw_9
    Snippet: We also designed a second pool of 171 multiplex PCR primers. These primers overlap with those in the previous 172-primer pool. Together, these two overlapping pools of primers deliver full coverage of the entire virus genome. If using both pools in detection, the calculated detection limit is 0.29 copies at 95% probability......
    Document: We also designed a second pool of 171 multiplex PCR primers. These primers overlap with those in the previous 172-primer pool. Together, these two overlapping pools of primers deliver full coverage of the entire virus genome. If using both pools in detection, the calculated detection limit is 0.29 copies at 95% probability.

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