Author: Danielle E Anderson; Jin Cui; Qian Ye; Baoying Huang; Wenhong Zu; Jing Gong; Weiqiang Liu; So Young Kim; Biao Guo Yan; Kristmundur Sigmundsson; Xiao Fang Lim; Fei Ye; Peihua Niu; Xuming Zhou; Wenjie Tan; Lin-Fa Wang; Xu Tan
Title: Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy Document date: 2020_3_30
ID: 0l33i6s4_48
Snippet: For siRNA screen, siRNAs targeting 18,328 genes of P. alecto were arrayed in the 96-well 5 plates. The siRNAs were reverse transfected into PaKi cells, 48 h later, the cells were infected with 6 mumps virus. The virus infection level was measured at 48 h post infection by high content 7 imaging (Operetta) as previously described 5 . The siRNA targeted genes were ranked by robust Z 8 scores 6 , and the potential host factors (Z score < -3) or rest.....
Document: For siRNA screen, siRNAs targeting 18,328 genes of P. alecto were arrayed in the 96-well 5 plates. The siRNAs were reverse transfected into PaKi cells, 48 h later, the cells were infected with 6 mumps virus. The virus infection level was measured at 48 h post infection by high content 7 imaging (Operetta) as previously described 5 . The siRNA targeted genes were ranked by robust Z 8 scores 6 , and the potential host factors (Z score < -3) or restriction factors (Z score > 6) were 9
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