Selected article for: "assay sequencing and dna sequencing"
Author: MS Zinter; CC Dvorak; MY Mayday; K Iwanaga; NP Ly; ME McGarry; GD Church; LE Faricy; CM Rowan; JR Hume; ME Steiner; ED Crawford; C Langelier; K Kalantar; ED Chow; S Miller; K Shimano; A Melton; GA Yanik; A Sapru; JL DeRisi
Title: Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised Children
  • Document date: 2018_3_29
    • ID: 28nlawnb_15
    Snippet: Iterative optimizations demonstrated that mechanical homogenization of BAL using 0.5mm inert bashing beads for 5 cycles in a DNA/RNA shield without mycolytic enzymes was superior to all other approaches for isolation of microbial nucleic acid (Supplemental Text 7, Supplemental Figures 1a-d) . Aspergillus. In comparison, the optimized DNA sequencing assay was able to detect as few as 6.13.....
    Document: Iterative optimizations demonstrated that mechanical homogenization of BAL using 0.5mm inert bashing beads for 5 cycles in a DNA/RNA shield without mycolytic enzymes was superior to all other approaches for isolation of microbial nucleic acid (Supplemental Text 7, Supplemental Figures 1a-d) . Aspergillus. In comparison, the optimized DNA sequencing assay was able to detect as few as 6.13

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